In silico molecular and morphological analysis of rice blast resistant gene Pi-ta in Sri Lankan rice germplasm.

ABSTRACT:

Background

Pi-ta is a major blast resistant gene, introgressed from indica rice varieties. In this study, diversity of the Pi-ta gene of 47 Sri Lankan rice accessions was studied by bioinformatics, and the results were validated with molecular and disease reaction assays. Sequences of rice accessions at the locus Os12g0281300 were retrieved from Rice SNP-Seek Database, and the coding sequence of reference Pi-ta gene of cultivar Tetep (accession no. GQ918486.1) was obtained from GenBank. Comparisons were made at nucleotide, amino acid, and protein structure level, and the 3D models predicted using Phyre2 software were superimposed using TM-align software.

Results

In silico analysis revealed that 10 accessions possessed resistant allele of the Pi-ta gene. The remaining accessi

SUBMITTER: Terensan S 

PROVIDER: S-EPMC8531186 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature