Single-cell transcriptomics reveal the heterogeneity and dynamic of cancer stem-like cells during breast tumor progression.

ABSTRACT: Breast cancer stem-like cells (BCSCs) play vital roles in tumorigenesis and progression. However, the origin and dynamic changes of BCSCs are still to be elucidated. Using the breast cancer mouse model MMTV-PyMT, we constructed a single-cell atlas of 31,778 cells from four distinct stages of tumor progression (hyperplasia, adenoma/MIN, early carcinoma and late carcinoma), during which malignant transition occurs. We identified that the precise cell type of ER^low epithelial cell lineage gave rise to the tumors, and the differentiation of ER^high epithelial cell lineage was blocked. Furthermore, we discovered a specific signature with a continuum of gene expression profiles along the tumor progression and significantly correlated with clinical outcomes, and we also found

SUBMITTER: Jiang G 

PROVIDER: S-EPMC8531288 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature