Plasma EVs Display Antigen-Presenting Characteristics in Patients With Allergic Rhinitis and Promote Differentiation of Th2 Cells.

ABSTRACT:

Background

Allergic rhinitis (AR) is characterized by IgE-mediated mucosa response after exposure to allergens. Extracellular vesicles (EVs) are nano-size vesicles containing biological cargos for intercellular communications. However, the role of plasma EVs in pathogenesis of AR remains largely unknown.

Methods

Plasma EVs from patients with AR were isolated, quantified, and characterized. The expression of Der p 1 and antigen-presenting molecules on EVs was determined by Western blot, flow cytometry, or ELISA. PKH26- and CFSE (carboxyfluorescein succinimidyl ester)-stained AR-EVs were used to determine the uptake of EVs by CD4⁺T cells and their effects on CD4⁺T cell proliferation, respectively.

Results

Plasma EVs in healthy control (HC) and AR p

SUBMITTER: Fang SB 

PROVIDER: S-EPMC8531542 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature