Colonic Motility Is Improved by the Activation of 5-HT_2B Receptors on Interstitial Cells of Cajal in Diabetic Mice.

ABSTRACT:

Background & aims

Constipation is commonly associated with diabetes. Serotonin (5-HT), produced predominantly by enterochromaffin (EC) cells via tryptophan hydroxylase 1 (TPH1), is a key modulator of gastrointestinal (GI) motility. However, the role of serotonergic signaling in constipation associated with diabetes is unknown.

Methods

We generated EC cell reporter Tph1-tdTom, EC cell-depleted Tph1-DTA, combined Tph1-tdTom-DTA, and interstitial cell of Cajal (ICC)-specific Kit-GCaMP6 mice. Male mice and surgically ovariectomized female mice were fed a high-fat high-sucrose diet to induce diabetes. The effect of serotonergic signaling on GI motility was studied by examining 5-HT receptor expression in the colon and in vivo GI transit, colonic migrating motor complexes (CMMCs),

SUBMITTER: Jin B 

PROVIDER: S-EPMC8532042 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature