Chemogenetic stimulation of the G_i pathway in astrocytes suppresses neuroinflammation.

ABSTRACT: Engineered G protein-coupled receptors (GPCRs) are commonly used in chemogenetics as designer receptors exclusively activated by designer drugs (DREADDs). Although several GPCRs have been studied in astrocytes using a chemogenetic approach, the functional role of the astrocytic G_i pathway is not clear, as the literature is conflicting depending on the brain regions or behaviors investigated. In this study, we evaluated the role of the astrocytic G_i pathway in neuroinflammation using a G_i -coupled DREADD (hM4Di). G_i -DREADD was expressed in hippocampal astrocytes of a lipopolysaccharide (LPS)-induced neuroinflammation mouse model using adeno-associated viruses. We found that astrocyte G_i -DREADD stimulation using clozapine N-oxide (CNO)

SUBMITTER: Kim JH 

PROVIDER: S-EPMC8532135 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature