Imipenem Resistance Mediated by bla_OXA-913 Gene in Pseudomonas aeruginosa.

ABSTRACT: Treatment of infectious diseases caused by carbapenem-resistant Pseudomonas aeruginosa is becoming a greater challenge. This study aimed to identify the imipenem resistance mechanism in P. aeruginosa isolated from a dog. Minimum Inhibitory Concentration (MIC) was determined by the broth microdilution method according to the Clinical and Laboratory Standards Institute recommendations. We performed polymerase chain reaction and whole-genome sequencing to detect carbapenem resistance genes. Genomic DNA of P. aeruginosa K19PSE24 was sequenced via the combined analysis of 20-kb PacBio SMRTbell and PacBio RS II. Peptide-Peptide Nucleic Acid conjugates (P-PNAs) targeting the translation initiation region of bla_OXA-913 were synthesized. The isolate (K19PSE24)

SUBMITTER: Moon DC 

PROVIDER: S-EPMC8532623 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature