Morphology of the Spleen in Oreochromis niloticus: Splenic Subregions and the Blood-Spleen Barrier.

ABSTRACT: The spleen is a separate organ of the teleost, playing an essential role in immune reactions. The morphology of the spleen is different from the fish species. Little knowledge about the spleen structure and the blood splenic barrier (BSB) in Nile tilapia has been reported. To address this issue, we studied the histology of the spleen and the BSB in healthy Nile tilapia. The morphology of the spleen was observed, then H&E staining, modified Jame's staining, and ultrastructural techniques were performed to portion the spleen into three subregions and analyze the location of components and fibers. Thereafter, vital staining of Nile tilapia with Trypan blue was conducted to elucidate the composition and function of BSB. Histologically, the spleen could be divided into three subregions (inner,

SUBMITTER: He Y 

PROVIDER: S-EPMC8532917 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature