Surface Modulation of Graphene Oxide for Amidase Immobilization with High Loadings for Efficient Biocatalysis.

ABSTRACT: As a type of important and versatile biocatalyst, amidase immobilization on solid materials has received broad attention with its relatively easy procedure and available reusability. However, current porous supports have suffered from limited loadings, and it is highly desired to develop a new type of material with abundant space so as to ensure a high loading of amidase. Here, graphene oxide was adopted as the support for amidase immobilization, which showed the highest loading capacity for amidase (~3000 mg/g) to date. To the best of our knowledge, it is the first case of amidase immobilized on graphene oxide. Through surface modulation via reducing the contents of oxygen-containing functional groups, activity recovery of immobilized amidase increased from 67.8% to 85.3%. Moreover, surfa

SUBMITTER: Xu K 

PROVIDER: S-EPMC8533581 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature