In Vivo PET Imaging of Monocytes Labeled with [⁸⁹Zr]Zr-PLGA-NH₂ Nanoparticles in Tumor and Staphylococcus aureus Infection Models.

ABSTRACT: The exponential growth of research on cell-based therapy is in major need of reliable and sensitive tracking of a small number of therapeutic cells to improve our understanding of the in vivo cell-targeting properties. ¹¹¹In-labeled poly(lactic-co-glycolic acid) with a primary amine endcap nanoparticles ([¹¹¹In]In-PLGA-NH₂ NPs) were previously used for cell labeling and in vivo tracking, using SPECT/CT imaging. However, to detect a low number of cells, a higher sensitivity of PET is preferred. Therefore, we developed ⁸⁹Zr-labeled NPs for ex vivo cell labeling and in vivo cell tracking, using PET/MRI. We intrinsically and efficiently labeled PLGA-NH₂ NPs with [⁸⁹Zr]ZrCl₄. In vitro, [⁸⁹Zr]Zr-PLGA-N

SUBMITTER: Krekorian M 

PROVIDER: S-EPMC8533969 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature