Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification.

ABSTRACT: An extraordinary optical transmission fibre-optic surface plasmon resonance biosensing platform was engineered to improve its portability and sensitivity, and was applied to monitor the concentrations of monoclonal antibodies (Mabs). By refining the fabricating procedure and changing the material of the flow cell and the components of the optical fibre, the biosensor is portable and robust to external interference. After the implementation of an effective template cleaning procedure and precise control during the fabrication process, a consistent sensitivity of 509 ± 5 nm per refractive index unit (nm/RIU) was achieved. The biosensor can detect the Mab with a limit of detection (LOD) of 0.44 µg/mL. The results show that the biosensor is a potential tool for the rapid quantification of Mab

SUBMITTER: Ly TT 

PROVIDER: S-EPMC8534111 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature