Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras.

ABSTRACT: Autophagy is a physiological degradation process that removes unnecessary or dysfunctional components of cells. It is important for normal cellular homeostasis and as a response to a variety of stresses, such as nutrient deprivation. Defects in autophagy have been linked to numerous human diseases, including cancers. Cancer cells require autophagy to migrate and to invade. Here, we study the intracellular topology of this interplay between autophagy and cell migration by an interdisciplinary live imaging approach which combines micro-patterning techniques and an autophagy reporter (RFP-GFP-LC3) to monitor over time, during directed migration, the back-front spatial distribution of LC3-positive compartments (autophagosomes and autolysosomes). Moreover, by exploiting a genetically controlled

SUBMITTER: Singh MK 

PROVIDER: S-EPMC8534269 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature