Determination of Pyrrolizidine Alkaloids in Teas Using Liquid Chromatography-Tandem Mass Spectrometry Combined with Rapid-Easy Extraction.

ABSTRACT: This study developed an analytical method to determine pyrrolizidine alkaloids (PAs) in teas using liquid chromatography-tandem mass spectrometry combined with rapid-easy extraction. PAs were extracted with 40 mL of 0.05 M sulfuric acid in 50% methanol solution and cleaned up using Oasis MCX SPE cartridges. Chromatographic separation of 21 PAs was conducted on an X-Bridge C18 column with gradient elution. According to the AOAC official analysis methods, the developed method was verified to establish linearity, limits of detection, limits of quantification, accuracy, inter-day precision, and intra-day precision for each PA. Overall, the method showed excellent repeatability, sensitivity, and reproducibility. The verified method was applied to tea samples, including maté, lemon balm, fennel,

SUBMITTER: Kwon Y 

PROVIDER: S-EPMC8534422 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature