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Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice.

ABSTRACT: Bioprinting is an emerging additive manufacturing technique which shows an outstanding potential for shaping customized functional substitutes for tissue engineering. Its introduction into the clinical space in order to replace injured organs could ideally overcome the limitations faced with allografts. Presently, even though there have been years of prolific research in the field, there is a wide gap to bridge in order to bring bioprinting from "bench to bedside". This is due to the fact that bioprinted designs have not yet reached the complexity required for clinical use, nor have clear GMP (good manufacturing practices) rules or precise regulatory guidelines been established. This review provides an overview of some of the most recent and remarkable achievements for skin, heart, pancreas and cartilage bioprinting breakthroughs while highlighting the critical shortcomings for each tissue type which is keeping this technique from becoming widespread reality.

SUBMITTER: Di Piazza E

PROVIDER: S-EPMC8535210 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice.

Di Piazza Eleonora E Pandolfi Elisabetta E Cacciotti Ilaria I Del Fattore Andrea A Tozzi Alberto Eugenio AE Secinaro Aurelio A Borro Luca L

International journal of environmental research and public health 20211014 20

Bioprinting is an emerging additive manufacturing technique which shows an outstanding potential for shaping customized functional substitutes for tissue engineering. Its introduction into the clinical space in order to replace injured organs could ideally overcome the limitations faced with allografts. Presently, even though there have been years of prolific research in the field, there is a wide gap to bridge in order to bring bioprinting from "bench to bedside". This is due to the fact that b ...[more]

PMID: 34682564

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Project description:BackgroundJoint degeneration and large or complex bone defects are a significant source of morbidity and diminished quality of life worldwide. There is an unmet need for a functional implant with near-native biomechanical properties. The potential for their generation using 3D bioprinting (3DBP)-based tissue engineering methods was assessed. We systematically reviewed the current state of 3DBP in orthoregeneration.MethodsThis review was performed using PubMed and Web of Science. Primary research articles reporting 3DBP of cartilage, bone, vasculature, and their osteochondral and vascular bone composites were considered. Full text English articles were analyzed.ResultsOver 1300 studies were retrieved, after removing duplicates, 1046 studies remained. After inclusion and exclusion criteria were applied, 114 articles were analyzed fully. Bioink material types and combinations were tallied. Cell types and testing methods were also analyzed. Nearly all papers determined the effect of 3DBP on cell survival. Bioink material physical characterization using gelation and rheology, and construct biomechanics were performed. In vitro testing methods assessed biochemistry, markers of extracellular matrix production and/or cell differentiation into respective lineages. In vivo proof-of-concept studies included full-thickness bone and joint defects as well as subcutaneous implantation in rodents followed by histological and µCT analyses to demonstrate implant growth and integration into surrounding native tissues.ConclusionsDespite its relative infancy, 3DBP is making an impact in joint and bone engineering. Several groups have demonstrated preclinical efficacy of mechanically robust constructs which integrate into articular joint defects in small animals. However, notable obstacles remain. Notably, researchers encountered pitfalls in scaling up constructs and establishing implant function and viability in long term animal models. Further, to translate from the laboratory to the clinic, standardized quality control metrics such as construct stiffness and graft integration metrics should be established with investigator consensus. While there is much work to be done, 3DBP implants have great potential to treat degenerative joint diseases and provide benefit to patients globally.

Project description:BACKGROUND:Satisfactory therapeutic strategies for cartilaginous lesion repair do not yet exist. This creates a challenge for surgeons and biomedical engineers and leads them to investigate the role of bioprinting and tissue engineering as viable treatments through orthopedic surgery, plastic surgery, and otorhinolaryngology. Recent increases in related scientific literature suggest that bioprinted cartilage may develop into a viable solution. OBJECTIVE:The objectives of this review were to (1) synthesize the scientific advances published to date, (2) identify unresolved technical problems regarding human application, and (3) identify more effective ways for the scientific community to transfer their findings to clinicians. METHODS:This scoping review considered articles published between 2009 and 2019 that were identified through searching PubMed, Scopus, Web of Science, and Google Scholar. Arksey and O'Malley's five-step framework was used to delimit and direct the initial search results, from which we established the following research questions: (1) What do authors of current research say about human application? (2) What necessary technical improvements are identified in the research? (3) On which issues do the authors agree? and (4) What future research priorities emerge in the studies? We used the Cohen kappa statistic to validate the interrater reliability. RESULTS:The 13 articles included in the review demonstrated the feasibility of cartilage bioprinting in live animal studies. Some investigators are already considering short-term human experimentation, although technical limitations still need to be resolved. Both the use and manufacturing process of stem cells need to be standardized, and a consensus is needed regarding the composition of hydrogels. Using on-site printing strategies and predesigned implants may allow techniques to adapt to multiple situations. In addition, the predictive capacity of implant behavior may lead to optimal results. CONCLUSIONS:Cartilage bioprinting for surgical applications is nearing its initial use in humans. Current research suggests that surgeons will soon be able to replace damaged tissue with bioprinted material.

Project description:Most governmental initiatives in India, to leash down urban air pollution, have yielded little results till date, largely due to purely technocratic vision, which is shrouded by technological, economic, social, institutional, and political hardships. We present this reflective article on urban greenery, as a proposition to urban authorities (e.g., pollution regulators, environmental systems' managers, urban landscape planners, environmental policy makers), shift from purely technocratic way of thinking to thinking with nature, by strategic greening of urban spaces, for long-term air pollution prevention and control measures. To that end, we offer a meta-analysis of recent (post 2005) global literature using four-stage PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) approach. We open the narrative by briefing about main pollutant filtration mechanisms by trees, followed by cognitive aspects of species selection (e.g., deciduous vs. evergreen, air pollution tolerance index, environmental stressors). Till date, most Indian studies on urban greenery mostly but focused on physiological aspects of trees. Here, we draw attention of urban authorities to an equally compelling, but yet less explored, aspect: design criteria, with reference to two most common urban configurations, namely, street canyon and open road. With pictorial depictions, we enumerate various categories of street canyons and discuss aspect ratio (building height to street width) and various wind flow regimes (isolated roughness, wake interface, and skimming), that the urban authorities should be cognizant about to maximize pollutant removal efficiency. For open road, we discuss vegetation barriers, with special emphasis on canopy porosity/density functions. In the final sections, we reflect on a potential systems' thinking approach for on-ground implementation, comprising of revamping of urban forestry programs, research and development, community mobilization and stakeholder engagement, and strategic outreach. In addition, we emphasize on means to harness co-benefits of urban greenery, beyond mere pollutant removal, to garner support from urban residents' communities. Last but not the least, we also caution the urban authorities about the undesirable outcomes of urban greenery that will require more process-level research.

Dataset Information

Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice.

Publications

Bioprinting Technology in Skin, Heart, Pancreas and Cartilage Tissues: Progress and Challenges in Clinical Practice.

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