Effects of Manganese on Genomic Integrity in the Multicellular Model Organism Caenorhabditis elegans.

ABSTRACT: Although manganese (Mn) is an essential trace element, overexposure is associated with Mn-induced toxicity and neurological dysfunction. Even though Mn-induced oxidative stress is discussed extensively, neither the underlying mechanisms of the potential consequences of Mn-induced oxidative stress on DNA damage and DNA repair, nor the possibly resulting toxicity are characterized yet. In this study, we use the model organism Caenorhabditis elegans to investigate the mode of action of Mn toxicity, focusing on genomic integrity by means of DNA damage and DNA damage response. Experiments were conducted to analyze Mn bioavailability, lethality, and induction of DNA damage. Different deletion mutant strains were then used to investigate the role of base excision repair (BER) and dePARylat

SUBMITTER: Nicolai MM 

PROVIDER: S-EPMC8535284 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature