Building a three-dimensional model of early-stage zebrafish embryo brain.

ABSTRACT: We introduce a computational approach to build three-dimensional (3D) surface mesh models of the early-stage zebrafish brain primordia from time-series microscopy images. The complexity of the early-stage brain primordia and lack of recognizable landmarks pose a distinct challenge for feature segmentation and 3D modeling. Additional difficulty arises because of noise and variations in pixel intensity. We overcome these by using a hierarchical approach in which simple geometric elements, such as "beads" and "bonds," are assigned to represent local features and their connectivity is used to smoothen the surface while retaining high-curvature regions. We apply our method to build models of two zebrafish embryo phenotypes at discrete time points between 19 and 28 h post-fertilization and colle

SUBMITTER: Chang-Gonzalez AC 

PROVIDER: S-EPMC8535780 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature