Isolation of RNA from Acute Ischemic Stroke Clots Retrieved by Mechanical Thrombectomy.

ABSTRACT: Mechanical thrombectomy (MT) for large vessel acute ischemic stroke (AIS) has enabled biologic analyses of resected clots. While clot histology has been well-studied, little is known about gene expression within the tissue, which could shed light on stroke pathophysiology. In this methodological study, we develop a pipeline for obtaining useful RNA from AIS clots. A total of 73 clot samples retrieved by MT were collected and stored in RNALater and in 10% phosphate-buffered formalin. RNA was extracted from all samples using a modified Chemagen magnetic bead extraction protocol on the PerkinElmer Chemagic 360. RNA was interrogated by UV-Vis absorption and electrophoretic quality control analysis. All samples with sufficient volume underwent traditional qPCR analysis and samples with sufficie

SUBMITTER: Tutino VM 

PROVIDER: S-EPMC8536169 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature