Metabolic control analysis of L-tryptophan producing Escherichia coli applying targeted perturbation with shikimate.

ABSTRACT: L-tryptophan production from glycerol with Escherichia coli was analysed by perturbation studies and metabolic control analysis. The insertion of a non-natural shikimate transporter into the genome of an Escherichia coli L-tryptophan production strain enabled targeted perturbation within the product pathway with shikimate during parallelised short-term perturbation experiments with cells withdrawn from a 15 L fed-batch production process. Expression of the shikimate/H⁺-symporter gene (shiA) from Corynebacterium glutamicum did not alter process performance within the estimation error. Metabolic analyses and subsequent extensive data evaluation were performed based on the data of the parallel analysis reactors and the production process. Extracellular rates and intracellular metab

SUBMITTER: Schoppel K 

PROVIDER: S-EPMC8536597 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature