Altered gene expression in slc4a11^-/- mouse cornea highlights SLC4A11 roles.

ABSTRACT: SLC4A11 is a H⁺/NH₃/water transport protein, of corneal endothelial cells. SLC4A11 mutations cause congenital hereditary endothelial dystrophy and some cases of Fuchs endothelial corneal dystrophy. To probe SLC4A11's roles, we compared gene expression in RNA from corneas of 17-week-old slc4a11^-/- (n = 3) and slc4a11^+/+ mice (n = 3) and subjected to RNA sequencing. mRNA levels for a subset of genes were also assessed by quantitative real-time reverse transcription PCR (qRT RT-PCR). Cornea expressed 13,173 genes, which were rank-ordered for their abundance. In slc4a11^-/- corneas, 100 genes had significantly altered expression. Abundant slc14a1 expression, encoding the urea transporter UT-A, suggests a significant role in the cornea. The

SUBMITTER: Alvarez BV 

PROVIDER: S-EPMC8536660 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature