TBC1D10C is a cytoskeletal functional linker that modulates cell spreading and phagocytosis in macrophages.

ABSTRACT: Cell spreading and phagocytosis are notably regulated by small GTPases and GAP proteins. TBC1D10C is a dual inhibitory protein with GAP activity. In immune cells, TBC1D10C is one of the elements regulating lymphocyte activation. However, its specific role in macrophages remains unknown. Here, we show that TBC1D10C engages in functions dependent on the cytoskeleton and plasma membrane reorganization. Using ex vivo and in vitro assays, we found that elimination and overexpression of TBC1D10C modified the cytoskeletal architecture of macrophages by decreasing and increasing the spreading ability of these cells, respectively. In addition, TBC1D10C overexpression contributed to higher phagocytic activity against Burkholderia cenocepacia and to increased cell membrane tension. Furthermore, by pe

SUBMITTER: Villagomez FR 

PROVIDER: S-EPMC8536695 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature