Restriction-free cloning for molecular manipulation and augmented expression of banana bunchy top viral coat protein.

ABSTRACT: Banana bunchy top virus (BBTV) causing bunchy top disease, is one of the most devastating diseases of banana and plantain. All the six genomic components of isolates from different parts of the world have been well characterised, with most of the studies focusing on replicase gene and coat protein gene. Overexpression of coat protein (CP) in Escherichia coli system can contribute significantly in structural as well as immunological studies. In the present investigation, the full length BBTV CP was cloned to pGEX-4T-2 expression vector and overexpressed in various Escherichia coli strains to obtain high quality and quantity of the CP. An augmented overexpression and stability of recombinant coat protein was achieved by molecular manipulation of the clone by restriction-

SUBMITTER: Dilip D 

PROVIDER: S-EPMC8536813 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature