Do Lipids Influence Gastrointestinal Processing: A Case Study of Major Soybean Allergen Gly m 4.

ABSTRACT: Previously, we have demonstrated that Gly m 4, one of the major soybean allergens, could pass through the Caco-2 epithelial barrier and have proposed a mechanism of sensitization. However, it is not known yet whether Gly m 4 can reach the intestine in its intact form after digestion in stomach. In the present work, we studied an influence of various factors including lipids (fatty acids and lysolipids) on digestibility of Gly m 4. Using fluorescent and CD spectroscopies, we showed that Gly m 4 interacted with oleic acid and LPPG (lyso-palmitoyl phosphatidylglycerol), but its binding affinity greatly decreased under acidic conditions, probably due to the protein denaturation. The mimicking of gastric digestion revealed that Gly m 4 digestibility could be significantly reduced with the chang

SUBMITTER: Finkina EI 

PROVIDER: S-EPMC8537068 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature