Epstein-Barr Virus Promotes Tumor Angiogenesis by Activating STIM1-Dependent Ca²⁺ Signaling in Nasopharyngeal Carcinoma.

ABSTRACT: Epstein-Barr virus (EBV) promotes tumor angiogenesis in nasopharyngeal carcinoma (NPC) by activating store-operated Ca²⁺ entry. Since such entry has been linked to stromal interaction molecule 1 (STIM1), we examined whether the virus acts via STIM1-dependent Ca²⁺ signaling to promote tumor angiogenesis in NPC. STIM1 expression was detected in NPC cell lines HK1 and CNE2 that were negative or positive for EBV. STIM1 was knocked down in EBV-positive cells using recombinant lentivirus, then cytosolic Ca²⁺ levels were measured based on fluorescence resonance energy transfer. Cells were also exposed to epidermal growth factor (EGF), and secretion of vascular endothelial growth factor (VEGF) was measured using an enzyme-linked immunosorbent assay. Endothelial tub

SUBMITTER: Ye J 

PROVIDER: S-EPMC8537240 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature