Egr1 Gene Expression as a Potential Biomarker for In Vitro Prediction of Ocular Toxicity.

ABSTRACT: Animal models are used for preclinical toxicity studies, and the need for in vitro alternative methods has been strongly raised. Our study aims to elucidate the potential mechanism of change in EGR1 expression under situations of toxic injury and to develop an Egr1 promoter-luciferase gene reporter assay for an in vitro alternative method for toxicity prediction in drug discovery. We first found an increase in early growth response-1 (EGR1) mRNA/protein expressions in the liver and kidney of cisplatin-treated injured rats. Additionally, the EGR1 protein level was also elevated under situations of ocular injury after sodium lauryl sulfate (SLS) eye drops. These in vivo observations on injury-related EGR1 induction were confirmed by in vitro studies, where human corneal epithelial cells were

SUBMITTER: Hwang DB 

PROVIDER: S-EPMC8537669 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature