Comparison of PCR versus PCR-Free DNA Library Preparation for Characterising the Human Faecal Virome.

ABSTRACT: The human intestinal microbiota is abundant in viruses, comprising mainly bacteriophages, occasionally outnumbering bacteria 10:1 and is termed the virome. Due to their high genetic diversity and the lack of suitable tools and reference databases, the virome remains poorly characterised and is often referred to as "viral dark matter". However, the choice of sequencing platforms, read lengths and library preparation make study design challenging with respect to the virome. Here we have compared the use of PCR and PCR-free methods for sequence-library construction on the Illumina sequencing platform for characterising the human faecal virome. Viral DNA was extracted from faecal samples of three healthy donors and sequenced. Our analysis shows that most variation was reflecting the individual

SUBMITTER: Hsieh SY 

PROVIDER: S-EPMC8537689 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature