Proteomic Analysis of Maternal Urine for the Early Detection of Preeclampsia and Fetal Growth Restriction.

ABSTRACT:

Background

To explore the use of maternal urine proteome for the identification of preeclampsia biomarkers.

Methods

Maternal urine samples from women with and without preeclampsia were used for protein discovery followed by a validation study. The targeted proteins of interest were then measured in urine samples collected at 20-24 and 30-34 weeks among nine women who developed preeclampsia, one woman with fetal growth restriction, and 20 women with uncomplicated pregnancies from a longitudinal study. Protein identification and quantification was obtained using liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Results

Among the 1108 urine proteins quantified in the discovery study, 21 were upregulated in preeclampsia and selected for validation. Nineteen (90%)

SUBMITTER: Bujold E 

PROVIDER: S-EPMC8537852 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature