Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations.

ABSTRACT: Femtosecond laser pulses have been successfully used for film-free single-cell bioprinting, enabling precise and efficient selection and positioning of individual mammalian cells from a complex cell mixture (based on morphology or fluorescence) onto a 2D target substrate or a 3D pre-processed scaffold. In order to evaluate the effects of higher pulse durations on the bioprinting process, we investigated cavitation bubble and jet dynamics in the femto- and picosecond regime. By increasing the laser pulse duration from 600 fs to 14.1 ps, less energy is deposited in the hydrogel for the cavitation bubble expansion, resulting in less kinetic energy for the jet propagation with a slower jet velocity. Under appropriate conditions, single cells can be reliably transferred with a cell survival rat

SUBMITTER: Zhang J 

PROVIDER: S-EPMC8538086 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature