Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.

ABSTRACT: Feline coronavirus (FCoV) is endemic in cat populations worldwide. Persistently, subclinically infected cats play a significant role in spreading the infection. Testing fecal samples of cats may facilitate efforts to decrease the viral burden within a population. Real-time RT-PCR is highly sensitive and specific for the detection of FCoV but must be performed in a fully equipped laboratory. A simple and accurate assay is needed to identify FCoV at the point-of-need. The aim of this study was to develop a rapid FCoV detection assay based on isothermal amplification technology, i.e., reverse transcription-recombinase polymerase amplification (RT-RPA). Primers were designed to target the highly conserved 3' untranslated region of the 7b gene. Running on a constant temperature of 42 °C, revers

SUBMITTER: Kobialka RM 

PROVIDER: S-EPMC8538120 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature