Knockdown of Vacuolar ATPase Subunit G Gene Affects Larval Survival and Impaired Pupation and Adult Emergence in Henosepilachna vigintioctopunctata.

ABSTRACT: The vATPase holoenzyme consists of two functional subcomplexes, the cytoplasmic (peripheral) V₁ and the membrane-embedded V₀. Both V₁ and V₀ sectors contain eight subunits, with stoichiometry of A₃B₃CDE₃FG₃H in V₁ and ac₈c'c"def(Voa1p) in V₀ respectively. However, the function of G subunit has not been characterized in any non-Drosophilid insect species. In the present paper, we uncovered that HvvATPaseG was actively transcribed from embryo to adult in a Coleopteran pest Henosepilachna vigintioctopunctata. Its mRNA levels peaked in larval hindgut and Malpighian tubules. RNA interference (RNAi)-mediated knockdown of HvvATPaseG significantly reduced la

SUBMITTER: Zeng J 

PROVIDER: S-EPMC8538789 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature