Best Molecular Tools to Investigate Coronavirus Diversity in Mammals: A Comparison.

ABSTRACT: Coronaviruses (CoVs) are widespread and highly diversified in wildlife and domestic mammals and can emerge as zoonotic or epizootic pathogens and consequently host shift from these reservoirs, highlighting the importance of veterinary surveillance. All genera can be found in mammals, with α and β showing the highest frequency and diversification. The aims of this study were to review the literature for features of CoV surveillance in animals, to test widely used molecular protocols, and to identify the most effective one in terms of spectrum and sensitivity. We combined a literature review with analyses in silico and in vitro using viral strains and archive field samples. We found that most protocols defined as pan-coronavirus are strongly biased towards α- and β-CoVs and show medium-low s

SUBMITTER: Drzewniokova P 

PROVIDER: S-EPMC8538982 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature