Raman Stable Isotope Probing of Bacteria in Visible and Deep UV-Ranges.

ABSTRACT: Raman stable isotope probing (Raman-SIP) is an excellent technique that can be used to access the overall metabolism of microorganisms. Recent studies have mainly used an excitation wavelength in the visible range to characterize isotopically labeled bacteria. In this work, we used UV resonance Raman spectroscopy (UVRR) to evaluate the spectral red-shifts caused by the uptake of isotopes (¹³C, ¹⁵N, ²H(D) and ¹⁸O) in E. coli cells. Moreover, we present a new approach based on the extraction of labeled DNA in combination with UVRR to identify metabolically active cells. The proof-of-principle study on E. coli revealed heterogeneities in the Raman features of both the bacterial cells and the extracted DNA after labeling with ¹³

SUBMITTER: Azemtsop Matanfack G 

PROVIDER: S-EPMC8539138 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature