Butyrate Alters Pyruvate Flux and Induces Lipid Accumulation in Cultured Colonocytes.

ABSTRACT: Butyrate is considered the primary energy source of colonocytes and has received wide attention due to its unique health benefits. Insight into the mechanistic effects of butyrate on cellular and metabolic function relies mainly on research in in-vitro-cultured cells. However, cells in culture differ from those in vivo in terms of metabolic phenotype and nutrient availability. For translation, it is therefore important to understand the impact of different nutrients on the effects of butyrate. We investigated the metabolic consequences of butyrate exposure under various culturing conditions, with a focus on the interaction between butyrate and glucose. To investigate whether the effects of butyrate were different between cells with high and low mitochondrial capacity, we cultured HT

SUBMITTER: Bekebrede AF 

PROVIDER: S-EPMC8539916 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature