Human Serum Extracellular Vesicle Proteomic Profile Depends on the Enrichment Method Employed.

ABSTRACT: The proteomic profiling of serum samples supposes a challenge due to the large abundance of a few blood proteins in comparison with other circulating proteins coming from different tissues and cells. Although the sensitivity of protein detection has increased enormously in the last years, specific strategies are still required to enrich less abundant proteins and get rid of abundant proteins such as albumin, lipoproteins, and immunoglobulins. One of the alternatives that has become more promising is to characterize circulating extracellular vesicles from serum samples that have great interest in biomedicine. In the present work, we enriched the extracellular vesicles fraction from human serum by applying different techniques, including ultracentrifugation, size-exclusion chromatography, an

SUBMITTER: Azkargorta M 

PROVIDER: S-EPMC8540106 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature