Full Neutralization of Centruroidessculpturatus Scorpion Venom by Combining Two Human Antibody Fragments.

ABSTRACT: A fundamental issue of the characterization of single-chain variable fragments (scFvs), capable of neutralizing scorpion toxins, is their cross-neutralizing ability. This aspect is very important in Mexico because all scorpions dangerous to humans belong to the Centruroides genus, where toxin sequences show high identity. Among toxin-neutralizing antibodies that were generated in a previous study, scFv 10FG2 showed a broad cross-reactivity against several Centruroides toxins, while the one of scFv LR is more limited. Both neutralizing scFvs recognize independent epitopes of the toxins. In the present work, the neutralization capacity of these two scFvs against two medically important toxins of the venom of Centruroides sculpturatus Ewing was evaluated. The results show

SUBMITTER: Riano-Umbarila L 

PROVIDER: S-EPMC8540208 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature