Progress and Challenges in Quantifying Carbonyl-Metabolomic Phenomes with LC-MS/MS.

ABSTRACT: Carbonyl-containing metabolites widely exist in biological samples and have important physiological functions. Thus, accurate and sensitive quantitative analysis of carbonyl-containing metabolites is crucial to provide insight into metabolic pathways as well as disease mechanisms. Although reversed phase liquid chromatography electrospray ionization mass spectrometry (RPLC-ESI-MS) is widely used due to the powerful separation capability of RPLC and high specificity and sensitivity of MS, but it is often challenging to directly analyze carbonyl-containing metabolites using RPLC-ESI-MS due to the poor ionization efficiency of neutral carbonyl groups in ESI. Modification of carbonyl-containing metabolites by a chemical derivatization strategy can overcome the obstacle of sensitivity; however,

SUBMITTER: Sun Y 

PROVIDER: S-EPMC8541004 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature