Activation of Drosophila melanogaster TRPA1 Isoforms by Citronellal and Menthol.

ABSTRACT:

Background

The transient receptor potential ankyrin 1 (TRPA1) cation channels function as broadly-tuned sensors of noxious chemicals in many species. Recent studies identified four functional TRPA1 isoforms in Drosophila melanogaster (dTRPA1(A) to (D)), but their responses to non-electrophilic chemicals are yet to be fully characterized.

Methods

We determined the behavioral responses of adult flies to the mammalian TRPA1 non-electrophilic activators citronellal and menthol, and characterized the effects of these compounds on all four dTRPA1 channel isoforms using intracellular Ca²⁺ imaging and whole-cell patch-clamp recordings.

Results

Wild type flies avoided citronellal and menthol in an olfactory test and this behavior was reduced in dTrpA1

SUBMITTER: Boonen B 

PROVIDER: S-EPMC8541009 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature