Generation of a Biomimetic Substitute of the Corneal Limbus Using Decellularized Scaffolds.

ABSTRACT: Patients with severe limbal damage and limbal stem cell deficiency are a therapeutic challenge. We evaluated four decellularization protocols applied to the full-thickness and half-thickness porcine limbus, and we used two cell types to recellularize the decellularized limbi. The results demonstrated that all protocols achieved efficient decellularization. However, the method that best preserved the transparency and composition of the limbus extracellular matrix was the use of 0.1% SDS applied to the half-thickness limbus. Recellularization with the limbal epithelial cell line SIRC and human adipose-derived mesenchymal stem cells (hADSCs) was able to generate a stratified epithelium able to express the limbal markers p63, pancytokeratin, and crystallin Z from day 7 in the case of SIRC and

SUBMITTER: Sanchez-Porras D 

PROVIDER: S-EPMC8541096 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature