Development of a gene doping detection method to detect overexpressed human follistatin using an adenovirus vector in mice.

ABSTRACT:

Background

Gene doping is the misuse of genome editing and gene therapy technologies for the purpose of manipulating specific genes or gene functions in order to improve athletic performance. However, a non-invasive detection method for gene doping using recombinant adenoviral (rAdV) vectors containing human follistatin (hFST) genes (rAdV<hFST>) has not yet been developed. Therefore, the aim of this study was to develop a method to detect gene doping using rAdV<hFST>.

Methods

First, we generated rAdV<hFST> and evaluated the overexpression of the hFST gene, FST protein, and muscle protein synthesis signaling using cell lines. Next, rAdV<hFST> was injected intravenously or intramuscularly into mice, and whole blood was collected, and <

SUBMITTER: Yanazawa K 

PROVIDER: S-EPMC8541302 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature