High-level embryonic globin production with efficient erythroid differentiation from a K562 erythroleukemia cell line.

ABSTRACT: A reliable cell line capable of robust in vitro erythroid differentiation would be useful to investigate red blood cell (RBC) biology and genetic strategies for RBC diseases. K562 cells are widely utilized for erythroid differentiation; however, current differentiation methods are insufficient to analyze globin proteins. In this study, we sought to improve erythroid differentiation from K562 cells to enable protein-level globin analysis. K562 cells were exposed to a variety of reagents, including hemin, rapamycin, imatinib, and/or decitabine (known erythroid inducers), and cultured in a basic culture medium or erythropoietin-based differentiation medium. All single reagents induced observable erythroid differentiation with higher glycophorin A (GPA) expression but were insufficient to prod

SUBMITTER: Uchida N 

PROVIDER: S-EPMC8541692 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature