Increased cell-free fetal DNA release after apoptosis and sterile inflammation in human trophoblast cells.

ABSTRACT:

Problem

Cell-free fetal DNA (cffDNA) shed from the placenta can be detected in maternal blood and increases incrementally during gestation. Concentrations are further elevated with pregnancy complications. Specific activators of cffDNA release in such complications have not been identified. Here, we use trophoblast cells from early and term placenta to examine cffDNA release following apoptosis, infection, and sterile inflammatory stress.

Method of study

HTR8/SVneo cells were used to model first-trimester trophoblasts, and term cytotrophoblasts (CTBs) were isolated from placentae collected after uncomplicated deliveries. Trophoblasts were treated with varying concentrations of doxorubicin (DOX), lipopolysaccharide (LPS), or high-mobility group box protein 1 (HMGB1) for 18 h.

SUBMITTER: Kazemi NY 

PROVIDER: S-EPMC8541917 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature