CRISPR/Cas9-mediated genome editing directed by a 5S rRNA-tRNA^Gly hybrid promoter in the thermophilic filamentous fungus Humicola insolens.

ABSTRACT:

Background

Humicola insolens is a filamentous fungus with high potential of producing neutral and heat- and alkali-resistant cellulase. However, the genetic engineering tools, particularly the genome-editing tool, are scarce, hindering the study of cellulase expression regulation in this organism.

Results

Herein, a CRISPR/Cas9 genome-editing system was established in H. insolens based on a hybrid 5S rRNA-tRNA^Gly promoter. This system is superior to the HDV (hepatitis delta virus) system in genome editing, allowing highly efficient single gene destruction in H. insolens with rates of deletion up to 84.1% (37/44). With this system, a putative pigment synthesis gene pks and the transcription factor xyr1 gene were disrupted with high efficiency. Moreover, the extracel

SUBMITTER: Fan C 

PROVIDER: S-EPMC8542335 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature