Regulation of MUC6 Methylation Correlates with Progression of Gastric Cancer.

ABSTRACT:

Purpose

This study aimed to investigate the mechanistic downregulation of mucin 6 (MUC6) and its influence on the progression of gastric cancer (GC).

Materials and methods

The expression of MUC6 was examined in 40 GC patients. The methylation status of the MUC6 promoter region was investigated using GC cell lines and GC tissue specimens by immunohistochemistry and/or quantitative polymerase chain reaction (qPCR). MUC6 was knocked down in the gastric epithelial cells (GES-1) cell and overexpressed in the SGC7901 cell. The effects of MUC6 knockdown and overexpression on cell migration and invasion were examined using Transwell assays. The effects of demethylation and methylation on MUC6 expression were examined by western blot, qPCR, or double luciferase reporter assays.

Re

SUBMITTER: Shi D 

PROVIDER: S-EPMC8542475 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature