Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy.

ABSTRACT:

Introduction

Structural changes in the collagen II architecture of osteoarthritis (OA) are poorly understood, which is a large shortcoming in the early diagnosis of this disease. Though degradation can be simulated by enzymes including trypsin and bacterial collagenase, the specific structural features of each digestion and their relationship to naturally occurring OA remain unclear.

Experimental design

We used collagen sensitive/specific Second Harmonic Generation (SHG) microscopy in conjunction with optical scattering measurements to probe the resulting architecture changes in bovine knee cartilage upon trypsin and collagenase degradation. Image features extracted from SHG images were used to train a linear discriminant (LD) model capable of classifying enzymatic degradati

SUBMITTER: Jambor AN 

PROVIDER: S-EPMC8542598 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature