Changes in Expressions of Spermatogenic Marker Genes and Spermatogenic Cell Population Caused by Stress.

ABSTRACT: Many young adults are in a state of stress due to social and psychological pressures, which may result in male reproductive dysfunction. To provide new insight into this phenomenon, we investigated the effect of stress on the regulation of key genes and biological events in specific stages of spermatogenesis. After establishing rat stress models of different time durations, we observed pathological changes in testis through haematoxylin and eosin staining, and analysed gene expression in testis by RNA-seq, bioinformatic analysis, and reverse transcription qPCR (RT-qPCR). Immunohistochemistry (IHC) with the TissueFAXS quantitative imaging system was used to verify changes of different population of spermatogenic cells marked by differentially expressed marker genes. Our results showed that

SUBMITTER: Tian P 

PROVIDER: S-EPMC8542882 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature