Molecular Epidemiology and Characteristics of CTX-M-55 Extended-Spectrum β-Lactamase-Producing Escherichia coli From Guangzhou, China.

ABSTRACT: In recent years, the CTX-M-55 extended-spectrum β-lactamase (ESBL)-positive rate has gradually increased in the clinic. To identify the molecular epidemiology and characteristics of bla _{CTX-M -55}-positive isolates, a total of 374 non-repetitive ESBL-producing Escherichia coli strains were collected from patients in two hospitals in Guangzhou, and 89 bla _{CTX-M -55}-positive isolates were selected by CTX-M-1-group PCR amplification and confirmed by DNA sequencing. Whole-genome sequencing was used to analyze the resistance phenotype, plasmid types, phylogenetic relationships and genetic environment of the bla _{CTX-M -55} gene. Conjugation experiments and PCR were performed to confirm whether the plasmid

SUBMITTER: Zeng S 

PROVIDER: S-EPMC8542904 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature