Rapid Identification of the Tumor-Specific Reactive TIL Repertoire <i>via</i> Combined Detection of CD137, TNF, and IFNγ, Following Recognition of Autologous Tumor-Antigens.

ABSTRACT: Detecting the entire repertoire of tumor-specific reactive tumor-infiltrating lymphocytes (TILs) is essential for investigating their immunological functions in the tumor microenvironment. Current in vitro assays identifying tumor-specific functional activation measure the upregulation of surface molecules, de novo production of antitumor cytokines, or mobilization of cytotoxic granules following recognition of tumor-antigens, yet there is no widely adopted standard method. Here we established an enhanced, yet simple, method for identifying simultaneously CD8⁺ and CD4⁺ tumor-specific reactive TILs in vitro, using a combination of widely known and available flow cytometry assays. By combining the detection of intracellular CD137 and de novo

SUBMITTER: Draghi A 

PROVIDER: S-EPMC8543011 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature