Loop-Mediated Isothermal Amplification assays for on-site detection of the main sweetpotato infecting viruses.

ABSTRACT: Globally, Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV) occur frequently and in combination cause sweetpotato virus disease (SPVD). Many viral diseases are economically important and negatively impact the production and movement of germplasm across regions. Rapid detection of viruses is critical for effective control. Detection and quantification of viruses directly from sweetpotato remains a challenge. Current diagnostic tests are not sensitive enough to reliably detect viruses directly from the plant or require expensive laboratory equipment and expertise to perform. We developed a simple and rapid loop-mediated isothermal amplification (LAMP) assay for the detection of SPFMV, SPCSV and begomoviruses related to sweet potato leaf curl virus (SPL

SUBMITTER: Wanjala BW 

PROVIDER: S-EPMC8543070 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature