Characterization of a highly xylose tolerant β-xylosidase isolated from high temperature horse manure compost.

ABSTRACT:

Background

There is a continued need for improved enzymes for industry. β-xylosidases are enzymes employed in a variety of industries and although many wild-type and engineered variants have been described, enzymes that are highly tolerant of the products produced by catalysis are not readily available and the fundamental mechanisms of tolerance are not well understood.

Results

Screening of a metagenomic library constructed of mDNA isolated from horse manure compost for β-xylosidase activity identified 26 positive hits. The fosmid clones were sequenced and bioinformatic analysis performed to identity putative β-xylosidases. Based on the novelty of its amino acid sequence and potential thermostability one enzyme (XylP81) was selected for expression and further characterizatio

SUBMITTER: Ndata K 

PROVIDER: S-EPMC8543862 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature