Expression and Display of Glycoengineered Antibodies and Antibody Fragments with an Engineered Yeast Strain.

ABSTRACT: Interactions with cell surface receptors enhance the therapeutic properties of many important antibodies, including the low-affinity Fc γ Receptors (FcγRs). These interactions require proper processing of the immunoglobulin G Fc N-glycan, and eliminating the N-glycan abolishes binding, restricting antibody production to mammalian expression platforms. Yeasts, for example, generate extensively mannosylated N-glycans that are unsuitable for therapeutics. However, Fc with a specifically truncated N-glycan still engages receptors with considerable affinity. Here we describe the creation and applications of a novel Saccharomyces cerevisiae strain that specifically modifies the IgG1 Fc domain with an N-glycan consisting of a single N-acetylglucosamine residue. This strain displayed glycoengineer

SUBMITTER: Shenoy A 

PROVIDER: S-EPMC8544235 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature