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Tissue Adhesion-Anisotropic Polyrotaxane Hydrogels Bilayered with Collagen.

ABSTRACT: Hydrogels are promising materials in tissue engineering scaffolds for healing and regenerating damaged biological tissues. Previously, we developed supramolecular hydrogels using polyrotaxane (PRX), consisting of multiple cyclic molecules threaded by an axis polymer for modulating cellular responses. However, since hydrogels generally have a large amount of water, their adhesion to tissues is extremely weak. Herein, we designed a bilayered hydrogel with a PRX layer and a collagen layer (PRX/collagen hydrogel) to achieve rapid and strong adhesion to the target tissue. The PRX/collagen hydrogel was fabricated by polymerizing PRX crosslinkers in water with placement of a collagen sponge. The differences in components between the PRX and collagen layers were analyzed using Fourier transform infrared spectroscopy (FT-IR). After confirming that the fibroblasts adhered to both layers of the PRX/collagen hydrogels, the hydrogels were implanted subcutaneously in mice. The PRX hydrogel without collagen moved out of its placement site 24 h after implantation, whereas the bilayer hydrogel was perfectly adherent at the site. Together, these findings indicate that the bilayer structure generated using PRX and collagen may be a rational design for performing anisotropic adhesion.

SUBMITTER: Hakariya M

PROVIDER: S-EPMC8544508 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Tissue Adhesion-Anisotropic Polyrotaxane Hydrogels Bilayered with Collagen.

Hakariya Masahiro M Arisaka Yoshinori Y Masuda Hiroki H Yoda Tetsuya T Tamura Atsushi A Iwata Takanori T Yui Nobuhiko N

Gels (Basel, Switzerland) 20211013 4

Hydrogels are promising materials in tissue engineering scaffolds for healing and regenerating damaged biological tissues. Previously, we developed supramolecular hydrogels using polyrotaxane (PRX), consisting of multiple cyclic molecules threaded by an axis polymer for modulating cellular responses. However, since hydrogels generally have a large amount of water, their adhesion to tissues is extremely weak. Herein, we designed a bilayered hydrogel with a PRX layer and a collagen layer (PRX/coll ...[more]

PMID: 34698173

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Dataset Information

Tissue Adhesion-Anisotropic Polyrotaxane Hydrogels Bilayered with Collagen.

Publications

Tissue Adhesion-Anisotropic Polyrotaxane Hydrogels Bilayered with Collagen.

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