A genetically encoded sensor for measuring serotonin dynamics.

ABSTRACT: Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB_5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB_5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.

SUBMITTER: Wan J 

PROVIDER: S-EPMC8544647 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature