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A genetically encoded sensor for measuring serotonin dynamics.

ABSTRACT: Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB_5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB_5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.

SUBMITTER: Wan J

PROVIDER: S-EPMC8544647 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature

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A genetically encoded sensor for measuring serotonin dynamics.

Wan Jinxia J Peng Wanling W Li Xuelin X Qian Tongrui T Song Kun K Zeng Jianzhi J Deng Fei F Hao Suyu S Feng Jiesi J Zhang Peng P Zhang Yajun Y Zou Jing J Pan Sunlei S Shin Mimi M Venton B Jill BJ Zhu J Julius JJ Jing Miao M Xu Min M Li Yulong Y

Nature neuroscience 20210405 5

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB<sub>5-HT</sub>) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB<sub>5-HT</sub> detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provid ...[more]

PMID: 33821000

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